Antigens of Pasteurella tularensis: preparative procedures.
نویسنده
چکیده
Ether-water (EW) extraction of Pasteurella tularensis produced better antigens than five other chemical procedures. EW extracts produced from stationary-phase, liquid-grown, saline suspensions of strain SCHU S4 cells regularly induced agglutinin and precipitin formation in rabbits. Mice, guinea pigs, and monkeys also responded to EW extracts but with lower antibody levels. The use of strains of lower virulence, acetone-dried cells, organisms grown on a solid medium, and abbreviated extraction conditions all resulted in extracts with a diminished antigenicity, but logarithmic-phase and stationary-phase cells yielded equivalent EW extracts. The use of adjuvant, hyperimmunization, and large doses of antigen increased the precipitin responses of rabbits without appreciably altering the agglutinin response. By the appropriate combination of centrifugal fractionation of EW extracts, use of adjuvant, and vaccination schedule, rabbit antisera with either predominantly agglutinating or precipitating activities were obtained.
منابع مشابه
Immunogenic Substances in Culture Filtrates and Lysates of Pasteurella Tularensis.
Hatch, Melvin T. (University of Utah, Salt Lake City), and Paul S. Nicholes. Immunogenic substances in culture filtrates and lysates of Pasteurella tularensis. J. Bacteriol. 88:566-573. 1964.-Culture filtrates and lysates of Pasteurella tularensis were tested for immunogenicity in mice subsequently infected with either strain 425 or 425 F4G. The efficacy of the vaccines varied with dosage and w...
متن کاملImmunoadsorbent for the Isolation of Pasteurella tularensis-Specific Antibodies.
The Schu-5 strain of Pasteurella tularensis was cross-linked with tetra-azotized benzidine and employed for the isolation of purified specific antibodies. The isolation procedure increased the content of specifically precipitable antibodies at least 40-fold.
متن کاملWhole blood amino acid changes following respiratory-acquired Pasteurella tularensis infection in man.
متن کامل
Chemically defined medium for the growth of Pasteurella tularensis.
Chemically defined media for the growth of Pasteurella tularensis have been described by Tamura and Fleming (1949) and more recently by Giesken (1954), Mager et al. (1954), and Traub et al. (1955). Upon application of the latter medium (Traub et al.) for the growth of a virulent strain of P. tularensis, it was noted that the viable cell yields were markedly lower than yields previously obtained...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied microbiology
دوره 22 1 شماره
صفحات -
تاریخ انتشار 1971